Restriction enzymes
We will shortly have the capacity to generate a wide range of recombinant restriction enzymes for molecular cloning purposes, including EcoRI, NotI, XbaI, PstI and many more.
Our recombinant restriction enzymes (REs) are typically synthesised by introducing specific viral DNA into a variety of bacterial species. The bacterial cells are then cultured to the required quantity, homogenised and the enzymes extracted and purified.
Different REs are classified according to the recognition sequence, the activity sites they cleave either side of that site, and the cofactors they require to function.
Restriction enzymes are commonly used for a wide variety of molecular biology research purposes. These include:
Cloning
REs are commonly used to insert sequences into plasmid vectors during gene cloning and protein generation.
Genotyping
If a single-nucleotide polymorphism alters a restriction site within a gene allele, it is possible to determine its presence by digesting the sample with a relevant RE, and analysing the resultant bands using agarose gel electrophoresis. The distance between activity sites can pinpoint the presence or absence of a given gene.
Southern blotting
In a similar manner to genotyping, REs can be used to determine the number of gene polymorphism are present in a given individual genome.
Sequencing
REs can be used to digest a large DNA molecule into smaller fragments, which can then be sequenced. At Wel-bioAps, careful selection of the expression vector and the strain of bacteria used provides extremely low contamination rates and exceptional purity, at larger yield and with minimal variation between batches.
Our restriction enzymes all exhibit >99% activity in BSA buffer (at 37 degrees.) DNA can be digested safely overnight.
How to order
- We work closely with our customers to provide cost-effective custom-made proteins, peptides and enzymes that meet the most exacting requirements.
- To begin the process, please email orders@wel-bioaps.com with any special requirements you may have.
- One of our team of scientists will contact you as soon as possible to discuss your needs and provide details on the next steps.
